Moderate Intensity Physical Exercise Decreases Gastrocnemius Caspase-3 Expression on Mus musculus with Statin Treatment

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Junita Jeanne Paliman
Ratna Darjanti Haryadi
Rwahita Satyawati Darmanta
Willy Sandhika
Martha Kurnia Kusumawardani

Abstract

ABSTRACTIntroduction: Dyslipidemia is a lipid metabolism abnormality marked by increase or decrease of lipid fraction in the plasma. Statin or 3-Hydroxy-3-Methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor is an important dyslipidemia medication that may induce apoptosis of the skeletal muscle.Decrease of Bcl-2 and Bax ratio causing caspase-9 activation followed by caspase-3 activation occur in apoptosis. Moderate intensity physical exercise (MIPE) decreases DNA fragmentation, increases Bcl-2 protein level and decreases Bax transcript level.Methods: Thirty healthy BALB/C strain Mus musculus divided into 3 groups: first intervention group with statin only treatment (S), second intervention group with statin treatment plus MIPE using motorized treadmill (ST), and control (C) group with no statin and nor exercise. The duration of treatmentwas 28 days. Statin treatment dose was 2.06mg/kg bodyweight. Measurement caspase-3 was done by immunohistochemistry evaluation and the expression of capase-3 was calculated by cells count which expressing caspase-3.Results: There were more muscle cells expressing caspase-3 in the S group (47.00±12.92cells) than the C group (2.70±1.94 cells); p=0.000, the ST group (21.44±10.13 cells) than the C group (p=0.001), as well as S group and the ST group (p=0.000).Conclusion: Moderate intensity physical exercise can decrease caspase-3 expression in statin treated subject.Keywords: apoptosis, caspase-3, dyslipidemia, moderate intensity physical exercise, statin.

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Author Biographies

Junita Jeanne Paliman, Medical Staff

Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Universitas Airlangga, Dr Soetomo General Hospital, Surabaya, Indonesia

Ratna Darjanti Haryadi, Medical Staff

Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Universitas Airlangga, Dr Soetomo General Hospital, Surabaya, Indonesia

Rwahita Satyawati Darmanta, Medical Staff

Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Universitas Airlangga, Dr Soetomo General Hospital, Surabaya, Indonesia

Willy Sandhika, Medical Staff

Department of Anatomical Pathology, Faculty of Medicine.Universitas Airlangga, Dr SoetomoGeneral Hospital, Surabaya, Indonesia

Martha Kurnia Kusumawardani, Medical Staff

Department of Physical Medicine and Rehabilitation, Faculty of Medicine,Universitas Airlangga, DR. Soetomo General Hospital, Surabaya, Indonesia