Gastrocnemius Muscle Histopathology In Spastic Rats
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Published:
Jun 1, 2022
Keywords:
alizerin red staining, gastrocnemius muscle, hematoxylin-eosin staining, histopathology, spastic rats
Main Article Content
Abstract
ABSTRACT
Introduction: Spasticity caused by the injury of the central nervous system. Muscle with spasticity causes muscle fibers to change in size and distribution.
Methods: To analyze the structure of the spasticity of the muscles, study use the excisional biopsy of the gastrocnemius muscle of spastic rats. Experimental study was conducted on 42 spastic rats in 6 groups with different doses of calcium. Staining of gastrocnemius muscle tissue fibers using hematoxylin-eosin.Calcium staining using alizerin red.
Results: Hematoxylin-eosin staining shown the muscle cell nucleus, cytoplasm or endoplasm appears roseate. Analysis of the spastic condition found that the distance between the muscle fibers dilated and the diameter smaller. Calcium deposits appear in spastic muscle tissue, by purplish-blue. The pearson correlation test showed a significant correlation between muscle fiber distance and spasticity after intervention (p<0.05). The spearman correlation test showed a significant correlation between spasticity after intervention (p=0.008) and each changes in spasticity (p=0.015).
Conclusions: HE staining used to determine the arrangement of muscle tissue in spastic gastrocnemius muscles and alizerin red determined calcium deposits in spasticity muscle tissue. There is a significant correlation between the distance between muscle fibers with spasticity and between changes in spasticity after the intervention.Keywords: alizerin red staining, gastrocnemius muscle, hematoxylin-eosin staining, histopathology, spastic rats
Introduction: Spasticity caused by the injury of the central nervous system. Muscle with spasticity causes muscle fibers to change in size and distribution.
Methods: To analyze the structure of the spasticity of the muscles, study use the excisional biopsy of the gastrocnemius muscle of spastic rats. Experimental study was conducted on 42 spastic rats in 6 groups with different doses of calcium. Staining of gastrocnemius muscle tissue fibers using hematoxylin-eosin.Calcium staining using alizerin red.
Results: Hematoxylin-eosin staining shown the muscle cell nucleus, cytoplasm or endoplasm appears roseate. Analysis of the spastic condition found that the distance between the muscle fibers dilated and the diameter smaller. Calcium deposits appear in spastic muscle tissue, by purplish-blue. The pearson correlation test showed a significant correlation between muscle fiber distance and spasticity after intervention (p<0.05). The spearman correlation test showed a significant correlation between spasticity after intervention (p=0.008) and each changes in spasticity (p=0.015).
Conclusions: HE staining used to determine the arrangement of muscle tissue in spastic gastrocnemius muscles and alizerin red determined calcium deposits in spasticity muscle tissue. There is a significant correlation between the distance between muscle fibers with spasticity and between changes in spasticity after the intervention.Keywords: alizerin red staining, gastrocnemius muscle, hematoxylin-eosin staining, histopathology, spastic rats
Article Details
How to Cite
Marina Indriasari, MD,Phd, Ferial Hadipoetro, & Bambang Pontjosoeryanto. (2022). Gastrocnemius Muscle Histopathology In Spastic Rats. Indonesian Journal of Physical Medicine and Rehabilitation, 11(01), 41 - 49. https://doi.org/10.36803/ijpmr.v11i01.327
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Original Article
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3. Kudryashev M. Muscle cells need calcium ions. EMBO Reports 2020; 21(5): 1-11.
4. Indriasari M, Hardinsyah, Kustiyah L, Priosoeryanto BP, Idris FH. Determination of the dose of erythrosine B as a preparation for inducing the experimental rats to be spastic. Mod Clin Med Res 2019; 3(2):
9-12.
5. Pacharinsak C, Smith JC. Handbook of laboratory animal anesthesia and pain management rodents. Boca Raton: CRC Press; 2017. p. 2-9.
6. Indrawati A, Ardita CA. Teknik pembuatan preparat histologi dengan
pewarnaan hematoksilin eosin (Tugas akhir D3 kesehatan hewan). Yogyakarta: Universitas Gajah Mada; 2017. p. 11-24
7. Kumar JL, Kiernan JA. Pathology education guide special stains and H & E. 2nd Edition.
California: Dako; 2010. p. 11-158.
8. Dufee NEd. Comparative anatomy of the mouse and the rat: A color atlas and
text. Memphis: American Association for Laboratory Animal Science (AALAS); 2011. p. 267
9. Junqueira LC. Histologi dasar. Edisi ke-12. Jakarta: EGC; 2010. p. 21
10. Hestianah EP, Chairul Anwar, Kuncorojakti S, Yustinasari LR. Buku ajar histologi
Veteriner jilid 1. Surabaya: Departemen Anatomi Veteriner Universitas Airlangga; 2018. p. 5-35.
11. Adams SB. Musculoskeletal disorders and diseases in dogs. USA: MSD and the
MSD Veterinary Manual; 2018. p. 1-2